RT Journal Article
SR Electronic
T1 The Effect of Transcatheter Injections on Cell Viability and Cytokine Release of Mononuclear Cells
JF American Journal of Neuroradiology
JO Am. J. Neuroradiol.
FD American Society of Neuroradiology
SP 1488
OP 1492
DO 10.3174/ajnr.A2092
VO 31
IS 8
A1 El Khoury, R.
A1 Misra, V.
A1 Sharma, S.
A1 Cox, C.S.
A1 Walker, P.
A1 Grotta, J.C.
A1 Gee, A.
A1 Suzuki, S.
A1 Savitz, S.I.
YR 2010
UL http://www.ajnr.org/content/31/8/1488.abstract
AB BACKGROUND AND PURPOSE: Several studies suggest that various types of cellular therapies enhance recovery after stroke in animal models. IA-based delivery of cells to the brain is under investigation for stroke, but it is unknown whether cells are injured as a result of being injected through a catheter or exposed to iodinated contrast medium or solutions containing heparin. MATERIALS AND METHODS: We assessed the effect of catheterization with the Excelsior SL-10 catheter or exposure to heparin or iodine contrast on human bone marrow MNCs. Viability and cell injury were assessed by trypan blue exclusion, caspase-3 activity, and lipid peroxidation. Cellular function of MNCs was assessed by their production and release of VEGF, IL-10, and IGF-1. RESULTS: Flow rates of 10 million cells from 0.5 to 2 mL/min did not alter MNC viability; however, 5 mL/min of MNCs did reduce viability by 19%. Iodine and low-dose heparin exposure did not affect cell viability; however, high-dose heparin was cytotoxic. Catheter delivery at 2 mL/min did not affect levels of VEGF, IL-10, or IGF-1. CONCLUSIONS: MNCs do not appear to be damaged by heparin, iodine contrast, and the Excelsior SL-10 catheter at flow rates up to 2 mL/min. However, higher flow rates did reduce viability, and high-dose heparin did cause cell death. Cath-HypohypoxiaDMEMDulbecco's Modified Eagle's MediumELISAenzyme-linked immunosorbent assayFDAUS Food and Drug AdministrationH.D.high doseH-HypoxMNCs not catheterized but conditionedIAintra-arterialIGF-1insulin-like growth factor 1IL-10interleukin 10L.D.low doseMNCmononuclear cellN-Cathnonconditioned MNCsN-ContMNCs not catheterizedO.D.optical densityPBSphosphate-buffered salineVEGFvascular endothelial growth factor