Estimated sample sizes for 80% or 90% power at 2-sided 5% significance level to detect specific yearly rates of WBNAA change with K (≥3) equally spaced scans per subject over 2 years

Yearly Rate of Change, mM (%)^*	Sample Size (80% Power)				Sample Size (90% Power)
Yearly Rate of Change, mM (%)^*	K = 3	K = 4	K = 5	K = 6	K = 3	K = 4	K = 5	K = 6
0.25 (2%)^*	57	52	46	42	76	68	61	55
0.51 (4%)^*	16	15	13	12	21	19	17	16
0.77 (6%)^*	9	8	8	7	11	10	9	8
1.03 (8%)^*	6	6	5	5	7	7	6	6

* Yearly rate of millimoles in percentage assumes a mean baseline WBNAA concentration of 12.8 mmol/L.¹¹