Recent examples of applications of molecular neuroimaging in gene therapy
| Type of Vector | Location of Imaging Gene, Therapeutic Gene | Imaging Gene Linked to Delivery | Site of Vector | Animal Model | Imaging Method | Application | Ref |
|---|---|---|---|---|---|---|---|
| Adeno-associated viruses | In delivery vehicle | No | In utero | Mice | Fluc BLI | Evaluation of systemic spread of virus, including to brain, for potential to deliver therapeutic genes to ameliorate genetic diseases with perinatal morbidity and mortality | 38 |
| Lentivirus | In delivery vehicle | Yes | Intravenous | Mice | Fluc BLI | Evaluation of gene therapy for Fabry disease using gene for α-galactosidase A, including in brain | 39 |
| Nonviral Sleeping Beauty transposon | In target gliomas | No | Brain, intratumoral | Mice | Fluc BLI | Evaluation of antiangiogenesis gene therapy delivered in gliomas; tumor burden assessed with Fluc BLI | 40 |
| Adenovirus | In delivery vehicle | Yes | Brain, intratumoral | Rats | Fluc BLI | Evaluation of glioma gene therapy using yCD/5-FC | 41 |
Note:—Ref indicates reference; BLI, bioluminescence imaging.