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Research ArticleBRAIN

Reversible MR Changes in the Cat Brain after Cerebral Fat Embolism Induced by Triolein Emulsion

Chang H. Lee, Hak J. Kim, Hae G. Kim, Sang D. Lee, Suk M. Son, Yong W. Kim, Seung M. Kim and Choong K. Eun
American Journal of Neuroradiology June 2004, 25 (6) 958-963;
Chang H. Lee
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Hak J. Kim
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Hae G. Kim
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Sang D. Lee
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Suk M. Son
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Yong W. Kim
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Seung M. Kim
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Choong K. Eun
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    Fig 1.

    Fat globules.

    A, Photomicrograph of the fat emulsion. The fat globules are of variable size, mostly less than two times the size of red blood cells (arrow, original magnification ×40).

    B, An intravascular fat globule is pinkish-red on oil red O staining (original magnification ×200).

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    Fig 2.

    Images obtained in a cat in group 4: A indicates T2WIs; B, Gd-enhanced T1WIs; C, DWIs; D, ADC maps; E, light photomicrograph; F, electron photomicrograph. 1 indicates 1 hour after embolization; 2, 1 day; and 3, 7 days. At 1 hour, the embolized lesion in the left hemisphere appears hyperintense in A1, enhanced in B1, mildly hyperintense in C1, and isointense in D1. At 1 day, T2WI hyperintensity (A2) and contrast enhancement (B2) are substantially decreased and not evident at day 7 (A3, B3). After day 1, DWIs (C2, C3) and ADC maps (D2, D3) reveal isointensity of the lesion. In E, Light microscopy of the gray matter (top) and white matter (bottom) shows no evidence of demyelination (Luxol fast blue stain, original magnification ×100). In F, Electron microscopy of the gray matter shows no evidence of neuropil or interstitial swelling (original magnification ×3000).

Tables

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    TABLE 1:

    MR imaging results in cats as a function of time after embolization

    Imaging*HyperintensityIsointensityHypointensity
    1 h (n = 50)
     T1WI03911
     T2WI27230
     DWI3120
     ADC0150
    1 d (n = 43)
     T1WI03310
     T2WI12310
     DWI0150
     ADC1140
    4 d (n = 36)
     T1WI0342
     T2WI1350
     DWI0150
     ADC0150
    1 wk, (n = 29)
     T1WI0290
     T2WI0290
     DWI0150
     ADC0150
    2 wk (n = 14)
     T1WI0140
     T2WI0150
    3 wk (n = 7)
     T1WI070
     T2WI070
    • Note.—Enhancement on Gd-enhanced T1WIs was as follows: at 1 hour, n = 44; at 1 day, n = 12; at 4 days, n = 1; and at 1, 2, and 3 weeks, n = 0.

    • * DWI and ADC in group 4 cats (n = 15).

    • View popup
    TABLE 2:

    Electron microscopic findings in cats in groups 1–6

    GroupIntravascular Fat GlobuleDefect in Blood-Brain BarrierPerivascular Interstitial SwellingNeuronal Swelling
    1 (n = 7)7643
    2 (n = 7)7557
    3 (n = 7)7321
    4 (n = 15)12925
    5 (n = 7)6222
    6 (n = 7)6100
    Total (n = 50)45261518
    • View popup
    TABLE 3:

    Intravascular fat globules in cats in groups 1–6

    GroupMean Number per mm2*Mean Rank†
    1 (n = 7)3.6225.25
    2 (n = 7)1.4514.92
    3 (n = 7)4.4623.33
    4 (n = 15)5.6227.71
    5 (n = 7)4.1722.75
    6 (n = 7)2.1118.50
    • * Intravascular fat globules were determined by means of oil red O staining.

    • † P = .397, Kruskal-Wallis test.

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American Journal of Neuroradiology: 25 (6)
American Journal of Neuroradiology
Vol. 25, Issue 6
1 Jun 2004
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Chang H. Lee, Hak J. Kim, Hae G. Kim, Sang D. Lee, Suk M. Son, Yong W. Kim, Seung M. Kim, Choong K. Eun
Reversible MR Changes in the Cat Brain after Cerebral Fat Embolism Induced by Triolein Emulsion
American Journal of Neuroradiology Jun 2004, 25 (6) 958-963;

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Reversible MR Changes in the Cat Brain after Cerebral Fat Embolism Induced by Triolein Emulsion
Chang H. Lee, Hak J. Kim, Hae G. Kim, Sang D. Lee, Suk M. Son, Yong W. Kim, Seung M. Kim, Choong K. Eun
American Journal of Neuroradiology Jun 2004, 25 (6) 958-963;
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