Regional Distribution and Clinical Correlates of White Matter Structural Damage in Huntington Disease: A Tract-Based Spatial Statistics Study

Supplemental Online Materials

Files in this Data Supplement:

• Online Tables 1-6 and Figures 1-9 (PDF) -
  
  Online Table 1: VBM results for volumes of GM and WM in 15 patients with HD (P < .05 FDR corrected)
  
  Online Table 2: VBM results showing the significant (P < .05 FDR corrected) correlation between individual scores of motor deficit and cognitive tests and the areas of GM and WM losses in 15 HD gene carriers
  
  Online Table 3: TBSS results: areas of increased MD in 15 patients with HD versus 15 healthy controls
  
  Online Table 4: TBSS results: correlation between decreased FA and Motor Scale score in 15 patients with HD
  
  Online Table 5: TBSS results: correlation between decreased FA and Stroop Color-Word interference test score in 15 patients with HD
  
  Online Table 6: TBSS results: correlation between decreased FA and Symbol Digit test score in 15 patients with HD
  
  Online Figure 1: VBM analysis in HD gene carriers vs healthy controls. Maps of the t-value (voxel analysis at p < 0.05 corrected for multiple comparisons) represented as glass brain (A,B) showing areas of GM (A) and WM (B) losses in HD gene carriers as compared to healthy controls. See Supplementary Table 1 for details.
  
  Online Figure 2: TBSS analysis of FA, λ||, �λ⊥ and MD maps in HD gene carriers vs healthy controls. Maps of the t-value (t >3 with p < 0.05 corrected for multiple comparisons) at a mid-ventricular level showing in red the areas of significantly decreased FA in the lateral portions of the posteriors corpus callosum and anterior fornix and in green the areas of significantly increased FA in the supralenticular frontal WM comprising the corticospinal tracts, anterior thalamic radiations and superior fronto-occipital fasciculus. A corresponding increase of the greatest eigenvalue is shown in pink by λ|| map, while the λ⊥ map shows its increase in sky blue only in the areas of decreased FA and elsewhere. The map of MD shows in yellow the areas of increased MD which are widespread and corresponds to those of altered FA and beyond.
  
  Online Figure 3: TBSS analysis of MD maps. Maps of the t-value (t >3 with p < 0.05 corrected for multiple comparisons) showing in yellow the areas of significantly increased in WM in HD gene carriers as compared to controls. These include multiple association tracts and the corpus callosum. Note the involvement of arciform fibres and of the cerebral peduncles (B,C) (colour bar indicates 1-p values).
  
  Online Figure 4: TTBSS correlation results in HD gene carriers. Blue shows the areas where FA inversely correlates (t >3 with p < 0.05 corrected for multiple comparisons) with the motor scale of the UHDRS. Note the predominant location within (head, body and splenium) (U) and beside the corpus callosum (colour bar indicates 1-p values) of the significant clusters.
  
  Online Figure 5: TBSS correlation results in HD gene carriers. Blue shows the areas where FA correlates (t >3 with p < 0.05 corrected for multiple comparisons) with the Stroop test results. Note the restriction of the areas to the frontal lobes and to the anterior portion of the corpus callosum body (U) �(colour bar indicates 1-p values).
  
  Online Figure 6: TBSS correlation results in HD gene carriers. Blue shows the areas where FA correlates (t >3 with p < 0.05 corrected for multiple comparisons) with the individual scores in the Symbol Digit test (colour bar indicates 1-p values). The clusters in the corpus callosum (U) are localized in the anterior and posterior portion of the body and in the head.
  
  Online Figure 7: TBSS correlation results in HD gene carriers. Green shows the areas where MD correlates (t >3 with p < 0.05 corrected for multiple comparisons) with the individual motor score (colour bar indicates 1-p values). The clusters in the corpus callosum (U) are localized in the body.
  
  Online Figure 8: TBSS correlation results in HD gene carriers. Green shows the areas where MD inversely correlates (t >3 with p < 0.05 corrected for multiple comparisons) with the individual scores in the Verbal Fluency test (colour bar indicates 1-p values).
  
  Online Figure 9: TBSS correlation results in HD gene carriers. Green shows the areas where MD inversely correlates (t >3 with p < 0.05 corrected for multiple comparisons) with the individual scores in the Symbol Digit test (colour bar indicates 1-p values).