Article Figures & Data
Figures
- Fig 1.
Definition of ROIs in MR spectroscopy and APT images. A, Illustration of the ROI in MR spectroscopy. For all animals, the right basal ganglia were selected as the ROI. B and C, Illustrations of ROIs in APT images (the T2WI serves as reference for the selection of ROIs in this study). In the control and HIBI groups, the bilateral basal ganglia were selected as ROIs, as shown by the areas marked with a solid line in B and C.
- Fig 2.
Time-related changes of APT values of the basal ganglia. After HI insult, APT values immediately decrease and reach the lowest level at 0–2 hours; thereafter, the values gradually increase, recover to the level of the control group at 12–24 hours, and then continue increasing. They are higher than the control group level at 48–72 hours.
- Fig 3.
Time-related changes of lactate in the control and HIBI groups. After HI insult, lactate increases immediately, reaches maximal value at 2–6 hours, and thereafter decreases gradually; in the HIBI model group, lactate is similar to that of the control group at 48–72 hours after HI injury.
- Fig 4.
Coronal T1WI, T2WI, and APT scans at the basal ganglia area in the control and the model groups (2 hours, 4 hours, and 68 hours after hypoxia-ischemia reperfusion). The APT signal demonstrates the hypointensity at 2 hours after HI, and then the signal gradually increases.
- Fig 5.
Results of 1H-MR spectroscopy data at selected time points in sample data analyzed by LCModel software. A–D, 1H-MR spectroscopy spectral curves of the right basal ganglia analyzed by LCModel in the control group and the HIBI group at 2 hours, 4 hours, and 68 hours, respectively. At 2 and 4 hours after HI insult, the lactate peaks (1.2–1.4 ppm) are markedly elevated, showing an upright single-peak or double-peak change; at 68 hours, the lactate peak is lower but still higher than that of the control group.
- Fig 6.
Changes and correlation between APT values and lactate content in basal ganglia of the HIBI model. A, The time-related changes in APTw intensity and lactate content are depicted in the same graph (the red square represents the APTw intensity at each time point; the blue circle denotes the lactate content at each time point). B, The correlation between APTw intensity and lactate content (r = −0.79, P = .036).
Tables
APT and lactate measurements at different time points
Parameters Control Model Group 0–2 Hours 2–6 Hours 6–12 Hours 12–24 Hours 24–48 Hours 48–72 Hours APT (%) 0.50 ± 0.12 −0.46 ± 0.25 0.02 ± 0.14 0.26 ± 0.04 0.47 ± 0.09 0.80 ± 0.11 1.31 ± 0.43 Lactate 0.43 ± 1.30 3.78 ± 4.31 20.45 ± 5.28 13.07 ± 1.10 2.31 ± 1.55 0.16 ± 0.25 0.51 ± 0.86
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